This is yet another positive example of the effectiveness of this listserv.
A friend of mine needed specimens, I posted it to the list, folks stepped up,
and it resulted in a significant contribution. Email me off-list if you are
interested in a copy. I apologize for this belated message.
Niagara River, NY
PS- One of the A. melas still resides in an aquarium (alive) as a bit of an
office mascot-in-PSU-Behrend College....
J. Great Lakes Res. 31(4):482-491.
Internat. Assoc. Great Lakes Res., 2005
Use of mtDNA to Identify Genetic Introgression among Related Species of
David W. Hunnicutt, John Cingolani and Margaret A. Voss, The
Pennsylvania State University-in-Erie, The Behrend College, School of
Science, Erie, Pennsylvania, 16563
ABSTRACT: Brown bullheads (Ameiurus nebulosus) are known to hybridize
naturally with closely related black (Ameiurus melas) and yellow
bullhead (Ameiurus natalis) species. The presence of hybrid specimens
may obscure accurate ecological and toxicological studies, which may in
turn influence evaluations of aquatic ecosystem health. Thus, there is a
need for a reliable method to detect introgression in bullheads from
tissue samples collected in the field. Specimens from each bullhead
species were identified using taxonomic keys and descriptions of
morphological characteristics. The morphological identifications were
compared with molecular characters from a 431 bp of mtDNA control region
sequences. The results identified a single possible A. melas x A.
nebulosus hybrid individual out of 11 fish identified as having A.
nebulosus morphology from Presque Isle Bay, Erie, PA. Four additional
fish revealed possible gene introgression from A. melas to A. nebulosus.
We suggest that future studies making toxicological and ecological
comparisons examine possible introgression within the bioindicator
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